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Description |
pEASY ®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products. •5 minutes fast ligation of Taq-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing. •T7 promoter for in vitro transcription.
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Storage |
At -20°C for 9 months |
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Kit Content |
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Component |
CT101-01 |
CT101-02 |
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(20 rxns) |
(60 rxns) |
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pEASY ®-T1 Cloning Vector (10 ng/μl) |
20µl |
3×20 µl |
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Control Template (5 ng/μl) |
5µl |
5µl |
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Control Primers (10 μM) |
5µl |
5µl |
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M13 Forward Primer (10 μM) |
50µl |
150µl |
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M13 Reverse Primer (10 μM) |
50µl |
150µl |
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l |
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Please note: Comp cells are available separately, but not included in the kit.
References:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| A maize wall-associated kinase confers quantitative resistance to head smut | Nature Genetics | 29.648 | Weiliang Zuo et al. | 2014 Dec | The original link |
| An Adaptive Transposable Element Insertion in the Regulatory Region of the EO Gene in the Domesticated Silkworm, Bombyx mori | Molecular Biology and Evolution | 14.308 | Wei Sun, et al. | 2014 Oct | The original link |
| pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements | Life | 9.322 | Xiaoyu Xiang, et al. | 2015 Feb | The original link |
| Undifferentiated embryonic cell transcription factor-1 (UTF1) inhibits the growth of cervical cancer cells by transactivating p27 Kip1 | Carcinogenesis | 5.635 | Wu X L, Zheng P S. Department of Reproductive Medi | 2013 Jul | The original link |
| Differential regulation of taurine biosynthesis in rainbow trout and Japanese flounder | Sci Rep. | 5.578 | Xuan Wang, et al. | 2016 Feb | The original link |