pEASY®-Blunt3 Cloning Kit

CB301

Description pEASY ®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion. •5 minutes fast ligation of Pfu-amplified PCR products. •Ampicillin resistance gene for selection. •Easy blue/white selection. •T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. •T7 promoter and SP6 promoter for in vitro transcription. Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Storage Competent Cell at -70℃ for six months; others at -20℃ for 9 months Kit Content Component CB301-01 CB301-02 (20 rxns) (60 rxns) pEASY ®-Blunt3 Cloning Vector (10 ng/μl) 20 µl 3×20 µl Control Template (5 ng/μl) 5 µl 5 µl Control Primers (10 μM) 5 µl 5 µl M13 Forward Primer (10 μM) 50 µl 150 µl M13 Reverse Primer (10 μM) 50 µl 150 µl Please note: this kit does not include competent cells.