Size :50ul
Clone Number:8A1D11
Aliases:Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG
Product Type:Monoclonal Antibody
Immunogen Species:Human
UniProt ID:Q9H9S0
Immunogen:Recombinant Human Homeobox protein NANOG protein (1-305AA)
Raised in:Mouse
Species Reactivity:Human, Mouse, Rat
Tested Applications:ELISA, WB, ICC, IF, FC; Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200
Background:Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Clonality:Monoclonal
Isotype:IgG1
Purification Method:>95%, Protein G purified
Conjugate:Non-conjugated
Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form:Liquid
Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names:NANOG
Research Areas:Stem Cells