Human Ferritin (FE) ELISA Kit

AE43829HU

Species Reactivity:Human (Homo sapiens)

UniProt:N/A

Abbreviation:FE

Alternative Names:N/A

Application:ELISA

Range:5-160 ng/mL

Sensitivity:2.2 ng/mL

Intra-AssayCV:?5.3%

Inter-AssayCV:?7.5%

Recovery:0.79

Sample Type:Serum, Plasma, Other biological fluids

Detection Method:Sandwich

Analysis Method??:Quantitive

Test principle:This assay employs a two-site sandwich ELISA to quantitate FE in samples. An antibody specific for FE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FE bound in the initial step. The color development is stopped and the intensity of the color is measured.

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