HiPure Plant RNA Plus Kit

R415002

Introduction

This product is suitable for extracting total RNA from 50-150mg conventional plant or fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 25-30 minutes. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection, and in vitro translation.

See protocol details in manual:

Details

Advantages

• Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - several samples can be extracted in 25 minutes by column method
• Safe - no phenol chloroform extraction required
• Sensitive - RNA can be recovered at the level of PG
• Broad spectrum - various types of plant samples can be processed by diversity of operating procedures
• TCEP effectively breaks protein disulfide bonds, enhancing lysis
  • TCEP prevents the lysate from browning
• TCEP maintains RNA integrity by preventing oxidation
  • especially in plant tissues with high levels of oxidizing agents

When dealing with woody or uncommon samples, The HiPure Plant RNA Plus Kit (R415002) and EasyPure® Universal Plant Total RNA Kit (ER302) are recommended first. R415002 contains two polysaccharide/polyphenol lysis buffer, which are amongst the most universal reagents. While ER302 contains an DNase I solution, as well as a strong polysaccharide/polyphenol lysis buffer. 

HiPure Plant RNA Mini (R415102) is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.

 

Kit Contents

Contents	R415002	D415003
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
gDNA Filter Columns	50	250
2ml Collection Tubes	100	500
TCEP (1M)	0.29 g	5 x 0.29 g
Buffer EP	1.0 ml	5.0 ml
Buffer PSL	50 ml	250 ml
Buffer RW1	50 ml	250 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

 

Storage and Stability

Except TCEP, the kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.