The GB-Ruler™ 50bp ladder plus is ideal for determining the size of double-stranded DNA from 50 to 1000 base pairs. The ladder consists of 13 linear double-stranded fragments .The 250bp and 500bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 250bp and 500bp are 40ng. The 250bp and 500bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommended Loading
2-5 µl/Lane
Concentration
Typical Bands 100 ng/5µl
Other Bands 40 ng/5µl
Advised Electrophoresis Condition
8 cm, 3% Agarose Gel, 0.5×TBE, 5 V/cm, 1 h
Contents（bp）
50、100、150、200、250、300、400、500、600、700、800、900、1,000
Mix Concentration
128 ng/µl
Storage
Stable for 3 months at RT, for long-time storage, please store at -20℃.