GB-Amp™ Universal qPCR Master Mix

Q712-02

This product is a special premix for qPCR reaction using the intercalating dye method. The core component, Taq ProDNA Polymerase, is a new generation of hot-start polymerase modified by antibody method. It has many advantages such as strong specificity, high detection sensitivity and high amplification yield. With the combination of Buffer optimized for qPCR and specific enhancer, it is very suitable for qPCR reaction with high specificity and sensitivity. This kit contains Specific ROX Reference Dye, which is suitable for all qPCR instruments without adjusting the concentration of ROX on different instruments. This kit contains 2 × master mix that can be amplified by adding primers and template.

Product Features

1. Ultra high plateau value
2. Perfect balance between sensitivity and specificity
3. All platform universality

Key Data

Ultra high plateau value

Hela cDNA was used as template to amplify six different genes by Q712 and Green qPCR reagents of other brands (from supplier A, B, C, D).

Perfect balance between sensitivity and specificity

Fig. A shows eight 10-fold gradient dilutions of pUC19 plasmid and Fig. B shows the standard curve obtained according to the CT values in Fig. A. In Fig C, cDNA of HeLa cells was used as a template, six different genes were amplified under the same reaction conditions using Q712 and Green qPCR reagents of other brands (from supplier A, B, C, D).

All platform universality

Q712 was used to amplify EGFR gene on different qPCR instruments (Type: ABI Stepone, ABI QuantStudio 3, and Roche LightCycler 480) with excellent quantitative results.

Components

Components	Q712-02 (500 rxns/20 μl reaction)	Q712-03 (2,500 rxns/20 μl reaction)
2 × GB-Amp™ Universal Green qPCR Master Mix*	4 × 1.25 ml	5 × Q712-02

* It contains dNTP, Mg2⁺, Taq Pro DNA Polymerase, Thiazole Green I and Specific ROX Reference Dye, etc.

So, we have optimized this master mixture, so that you do not have to worry about whether to spike the reference dye, or what level of the reference dye to spike, while at the same time, enjoying the high performance level of this Green Master qPCR Mix!