FlyCut® SpeI

JS601-01

Product Details

FlyCut^® SpeI is expressed and purified from E.coli that carries the recombinant SpeI gene. The molecular weight is 21.7 kDa, with the recognition site at A^CTAGT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...A↓CTAGT...3’
3’...TGATC↑A...5’

See manual for more details on JS601:

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C 

Shipping
Ice/gel packs (4℃)

Product Contents

Component	JS601-01	JS601-02
FlyCut® SpeI	250 units	2×250 units
10×FlyCut® Buffer	125 μl	250 μl
10×DNA Loading Buffer	0.5 ml	1 ml