FlyCut® SmaI is expressed and purified from E.coli that carries the recombinant SmaI gene. The molecular weight is 29.7 kDa, with the recognition site at CCC^GGG The reaction is conducted for 5-15 minutes at 25oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
Recognition Site
5’...CCC↓GGG...3’
3’...GGG↑CCC...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JS501-01 | JS501-02 |
| FlyCut® SmaI | 1,000 units | 2×1,000 units |
| 10×FlyCut® Buffer | 250 μl | 500 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |