
FlyCut® ScaI is expressed and purified from E.coli that carries the recombinant ScaI gene. The molecular weight is 27.8 kDa, with the recognition site at AGT^ACT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...AGT↓ACT...3’
3’...TCA↑TGA...5’
See manual for more details on JS401:
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C
Shipping
Ice/gel packs (4℃)
Component | JS401-01 | JS401-02 |
FlyCut® ScaI | 500 units | 2×500 units |
10×FlyCut® Buffer | 125 μl | 250 μl |
10×DNA Loading Buffer | 0.5 ml | 1 ml |
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