FlyCut® PvuI

JP201-01

Product Details

FlyCut^® PvuI is expressed and purified from E.coli that carries the recombinant PvuI gene. The molecular weight is 30.8 kDa, with the recognition site at CGAT^CG The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.

Recognition Site
5’...CGAT↓CG...3’
3’...GC↑TAGC...5’

See manual for more details on JP201:

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C

Shipping
Ice/gel packs (4℃)

Product Contents

Component	JP201-01	JP201-02
FlyCut® PvuI	250 units	2×250 units
10×FlyCut® Buffer	125 μl	250 μl
10×DNA Loading Buffer	0.5 ml	1 ml