
FlyCut® PstI is expressed and purified from E.coli that carries the recombinant PstI gene. The molecular weight is 38.3 kDa, with the recognition site at CTGCA^G. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...CTGCA↑G...3’
3’...G↑ACGTC...5’
See manual for more details on JP101:
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C
Shipping
Ice/gel packs (4℃)
Component | JP101-01 | JP101-02 |
FlyCut® PstI | 5,000 units | 2×5,000 units |
10×FlyCut® Buffer | 1.5 ml | 3×1 ml |
10×DNA Loading Buffer | 0.5 ml | 1 ml |
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