FlyCut® NotI

JN401-01

Product Details

FlyCut^® NotI is expressed and purified from E.coli that carries the recombinant NotI gene. The molecular weight is 43.3 kDa, with the recognition site at GC^GGCCGC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...GC↓GGCCGC...3’
3’...CGCCGG↑CG...5’

See manual for more details on JN401:

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C

Shipping
Ice/gel packs (4℃)

Product Contents

Component	JN401-01	JN401-02
FlyCut® NotI	250 units	2×250 units
10×FlyCut® Buffer	125 μl	250 μl
10×DNA Loading Buffer	0.5 ml	1 ml