FlyCut® NheI

JN301-01

Product Details

FlyCut^® NheI is expressed and purified from E.coli that carries the recombinant NheI gene. The molecular weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...G↓CTAGC...3’
3’...CGATC↑G...5’

See manual for more details on JN301:

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C

Shipping
Dry ice (-70 ℃)

Product Contents

Component	JN301-01	JN301-02
FlyCut® NheI	500 units	2×500 units
10×FlyCut® Buffer	125 μl	250 μl
10×DNA Loading Buffer	0.5 ml	1 ml