FlyCut® NcoI

JN101-01

Product Details

FlyCut^® NcoI is expressed and purified from E.coli that carries the recombinant NcoI gene. The molecular weight is 32.3 kDa, with the recognition site at C^CATGG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...C↓CATGG...3’
3’...GGTAC↑C...5’

See manual for more details on JN101:

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C

Shipping
Ice/gel packs (4℃)

Product Contents

Component	JN101-01	JN101-02
FlyCut® NcoI	500 units	2×500 units
10×FlyCut® Buffer	125 μl	250 μl
10×DNA Loading Buffer	0.5 ml	1 ml