FlyCut® HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. The molecular weight is 35.8kDa, with the recognition site at A^AGCTT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...A↓AGCTT...3’
3’...TTCGA↑A...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JH101-01 | JH101-02 |
| FlyCut® HindIII | 5,000 units | 2×5,000 units |
| 10×FlyCut® Buffer | 2×1 ml | 3×1 ml |
| 10×DNA Loading Buffer | 1 ml | 1 ml |