FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.
Recognition Site
5’...G↓AATTC...3’
3’...CTTAA↑G...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JE201-01 | JE201-02 |
| FlyCut® EcoRI | 5,000 units | 2×5,000 units |
| 10×FlyCut® Buffer | 2×1 ml | 3×1 ml |
| 10×DNA Loading Buffer | 1 ml | 1 ml |