FlyCut® EagI is expressed and purified from E.coli that carries the recombinant EagI gene. The molecular weight is 35.1 kDa, with the recognition site at C^GGCCG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Recognition Site
5’...C↓GGCCG...3’
3’...GCCGG↑C...5’
See manual for more details on JE101:
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C
Shipping
Ice/gel packs (4℃)
| Component | JE101-01 | JE101-02 |
| FlyCut® EagI | 250 units | 2×250 units |
| 10×FlyCut® Buffer | 125 μl | 250 μl |
| 10×DNA Loading Buffer | 0.5 ml | 1 ml |