
Species Reactivity:Chicken (Gallus)
UniProt:N/A
Abbreviation:IBDV-VP2-Ab
Alternative Names:N/A
Application:ELISA
Range:Detection antibody
Sensitivity:Request Information
Intra-AssayCV:?5.2%
Inter-AssayCV:?7.5%
Recovery:0.94
Sample Type:Serum, Plasma, Other biological fluids
Detection Method:Sandwich
Analysis Method??:Qualitative
Test principle:This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IBDV-VP2-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated IBDV-VP2-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled IBDV-VP2-Ab and unlabeled IBDV-VP2-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IBDV-VP2-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview:
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
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