The 10bp DNA Ladder is ideal for determining the size of double-stranded DNA from 80 to 300 base pairs. The ladder consists of 18 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommended Loading
2-5 µl/Lane
Concentration
Typical Bands 100 ng/5µl
Other Bands 40 ng/5µl
Advised Electrophoresis Condition
1 µl/Lane, 20 cm ,10%Polyacrylamide Gel, 1×TBE, 8 V/cm, 3h.
Contents(bp)
80、90、100、110、120、130、140、150、160、170、180、190、200、220、240、260、280、300
Mix Concentration
168 ng/µl
Storage
Stable for 3 months at RT, for long-time storage, please store at -20℃.