TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

AT341-01

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TransScript^® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )-AT341

• Specification：
• 50 rxns×20 µl system
• 100 rxns×20 µl system
• 500 rxns×20 µl system

Product Details

The mastermix provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR (not for regular end-point PCR). qPCR mastermix is not included with product.

• Simultaneous genomic DNA removal and cDNA synthesis. 
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.
  

This mastermix synthesizes first-strand cDNA for qPCR, it does not contain the components for a qPCR reaction. 

PerfectStart® Universal Green qPCR SuperMix (Cat.#AQ602) sold separately from AT341.

For a true one-step cDNA synthesis and qPCR supermix (qRT-PCR), Please see our TransScript® II Green One-Step qRT-PCR SuperMix (Cat.#AQ311).

 

See manual for more details on AT341:

Applications

Multiple copy and low copy gene detection

Storage

at -20 ℃

Shipping

Ice/Gel packs (4℃)

 

Product contents

Component	AT341-01 (50 rxns )	AT341-02 (100 rxns )	AT341-03 (500 rxns )
5×TransScript® All-in-One SuperMix for qPCR	200 μl	400 μl	5×400 μl
5×TransScript® All-in-One No-RT Control SuperMix for qPCR	20 μl	40 μl	200 μl
gDNA Remover	50 μl	100 μl	5×100 μl
RNase-free Water	1 ml	2×1 ml	2×5 ml

Citations

Han X , Wang R , Zhou Y , et al. Mapping the Mouse Cell Atlas by Microwell-Seq[J]. Cell, 2018, 172(5):1091-1107.e17.

Tian C , Zhang X , He J , et al. An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation[J]. Molecular Systems Biology, 2014, 10(10):755-755.

Wang J , Zhang Y , Hou J , et al. Ube2s regulates Sox2 stability and mouse ES cell maintenance[J]. Cell death and differentiation, 2015, 23(2).

Zhang L , Zhao X , Zhang G , et al. Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0[J]. Scientific Reports, 2016, 6:20761.

Li P , Shi M L , Shen W L , et al. Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions[J]. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2017, 1860(8):885-893.

Customer Reviews

Great product, competitve price, fantastic customer service

The cDNA synthesis kit works great and it has a very competitive price. We use half a reaction and it still working great! Customer service is fantastic, they are always ready to help.