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Description |
TransNGSTM Library Quantification Kit for Illumina® provides all reagents needed for qPCR based quantification of DNA libraries prepared for Illumina next generation sequencing platforms. Five linear DNA Standards with different GC contents are provided for customers to choose from. The kit also contains PCR primers which target the P5 and P7 adaptor sequences and TransNGSTM Library Quantification qPCR SuperMix (2×) for library amplifications. |
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Hightlights |
Five sets of linear DNA Standards (25%, 37.5%, 50%, 62.5%, and 75% of GC contents) to choose from Each DNA Standard contains six 10-fold dilution series of a linear 420 bp template TransNGSTM Library Quantification qPCR SuperMix (2×) enables accurate library amplification with various GC contents. |
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Storage |
at -20°C in dark for one year |
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Kit Content |
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Component |
KQ101-01 (100 rxns) |
KQ101-02 (500 rxns) |
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TransNGSTM Library Quantification qPCR SuperMix (2×) |
1 ml |
5×1 ml |
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Library Quantification Primer Mix (20×) |
100 μl |
500 μl |
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DNA Standards (S1-S6) |
24 μl each |
120 μl each |
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Library Dilution Buffer (10×) |
1 ml |
5 ml |
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Passive Reference Dye (50×) |
40 μl |
200 μl |
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ddH2O |
10 ml |
50 ml |