Recombinant Mycobacterium smegmatis DNA protection during starvation protein(dps)

CSB-EP314354MVW

>Several Other Sizes Are Also Available. Please Inquire. Default Size: 200ug

Updated Date: Stock Protein updated on 20170405

Research areas: Microbiology

Target / Protein: dps

Biologically active: Not Tested

Expression system: E.coli

Species of origin: Mycobacterium smegmatis

Delivery time: 3-7 business days

Uniprot ID: P0C558

AA Sequence: MTSFTIPGLSDKKASDVADLLQKQLSTYNDLHLTLKHVHWNVVGPNFIGVHEMIDPQVELVRGYADEVAERIATLGKSPKGTPGAIIKDRTWDDYSVERDTVQAHLAALDLVYNGVIEDTRKSIEKLEDLDLVSQDLLIAHAGELEKFQWFVRAHLESAGGQLTHEGQSTEKGAADKARRKSA

Tag info: N-terminal 6xHis-SUMO-tagged

Expression Region: 1-183aa

Protein length: Full Length

MW: 36.3 kDa

Alternative Name(s):

Relevance: Protects DNA from oxidative damage by sequestering intracellular Fe2+ ion and storing it in the form of Fe3+ oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe2+ ions, which prevents hydroxyl radical production by the Fenton reaction (By similarity). It protects DNA from hydroxyl radical-mediated cleavage. Binds DNA with no apparent sequence specificity without self-aggregation nor promotion of DNA condensation. Is unable to protect DNA from DNase-mediated cleavage.

Reference: "Bimodal protection of DNA by Mycobacterium smegmatis DNA-binding protein from stationary phase cells."Gupta S., Chatterji D.J. Biol. Chem. 278:5235-5241(2003)

Purity: Greater than 90% as determined by SDS-PAGE.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.