Size: 200ug. Other sizes are also available. Please Inquire.
In Stock: No
Lead time: 10-20 working days
Research Topic: Others
Uniprot ID: P43641
Gene Names: munIM
Organism: Mycoplasma sp.
AA Sequence: MENKVTAYSIYNKKAKKNTKVNPLDEVFPQLPRKKYQVIYADPPWDYGGKMQYDKSTIKSENEGFKRDIFISSASFKYPTLKLKELQQLDVPSITADDCILFMWTTGPQMANSILLGESWGFEYKTVAFVWDKMVHNPGRYTLSQTEFVLVFKKGKIPTPRGARNVRQLLQIHRGQHSEKPYAVIDGITKMFPALDKIELFARNNFVGWDNWGLEIPDNKIEIPTQGEIDENK
Expression Region: 1-233aa
Sequence Info: Full Length
Source: E.coli
Tag Info: N-terminal 6xHis-SUMO-tagged
MW: 42.9 kDa
Alternative Name(s): Adenine-specific methyltransferase MunI ;M.MunI
Relevance: This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease.
Reference: CAATTG-specific restriction-modification munI genes from Mycoplasma sequence similarities between R.MunI and R.EcoRI.Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A.Gene 142:1-8(1994)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage Buffer: Tris-based buffer，50% glycerol
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.