
Size:100ul
Host:Rabbit
Clonality:Polyclonal
Isotype:IgG
GeneName:POLR2A
GeneID:5430
Uniprot:P24928
Specificity:Phospho-Rpb1 CTD (Ser5/Ser1801) Antibody detects endogenous levels of Rpb1 CTD only when phosphorylated at Ser5/Ser1801.
Immunogen:A synthesized peptide derived from human Rpb1 CTD around the phosphorylation site of Ser5/Ser1801.
Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity:Human,Mouse,Rat
Prediction:
Application:WB,ELISA(peptide)
Dilution:WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Conjugate:Unconjugated
RRID:AB_2847012
An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide:AF3698-BP
Des# Blocking Peptide:Phospho-Rpb1 CTD (Ser5/Ser1801) Blocking peptide
Blocking Peptide Price:$549 CAD
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