HiPure Universal DNA Kit

D301802

Introduction

The HiPure Universal DNA Kit—a reliable and efficient solution for the quick extraction of total DNA from various clinical samples, such as tissue, cells, blood, saliva, swabs, blood spots, and semen. The extracted DNA is ready for immediate use in PCR, qPCR, Southern Blot analysis, and viral DNA testing.

Specifications

Features	Specifications
Main Functions	Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications	PCR, qPCR, southern bolt and virus detection, etc
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples
Sample amount	Solid tissue: 1-10mg; Anticoagulant blood: 200μl
Elution volume	≥20μl
Time per run	30 - 60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

This kit utilizes silica column-based purification technology. The process starts with the lysis and proteolytic digestion of the sample, releasing DNA into the lysate. The lysate is then transferred to an adsorption column where nucleic acids bind to the membrane. Proteins and other impurities are washed away, and the purified nucleic acid is eluted with a low-salt buffer (10 mM Tris, pH 9.0, 0.5 mM EDTA).

Advantages

• High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast - without separation of leukocytes, organic extraction or ethanol precipitation
• Simple - all nucleic acids can be obtained by direct digestion
• Wide applicability- handle a variety of liquid samples

Kit Contents

Contents	D301802	D301803
Purification Times	50	250
HiPure DNA Mini Columns I	50	250
2ml Collection Tubes	100	500
Buffer ATL	30 ml	150 ml
Buffer AL	30 ml	150 ml
Buffer GW1	22 ml	88 ml
Buffer GW2	12 ml	50 ml
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.