HiPure Plant RNA Mini Kit

R415102

Introduction

The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Advantages

• Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - several samples can be extracted in 25 minutes by column method
• Safe - no phenol chloroform extraction required
• Sensitive - RNA can be recovered at the level of PG
• Broad spectrum - various types of plant samples can be processed by diversity of operating procedures

See protocol details in manual:

When dealing with woody or uncommon samples, The HiPure Plant RNA Plus Kit (R415002) and EasyPure® Universal Plant Total RNA Kit (ER302) are recommended first. R415002 contains two polysaccharide/polyphenol lysis buffer, which are amongst the most universal reagents. While ER302 contains an DNase I solution, as well as a strong polysaccharide/polyphenol lysis buffer. 

HiPure Plant RNA Mini (R415102) is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.

 

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 150 mg plant using two columns (gDNA removed column)
Applications	RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology, DNA filtration technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic crops
Sample amount	≤150 mg
Elution volume	≥30μl
Time per run	≤25 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.

Kit Contents

Contents	R415102	D415103
Purification Times	50 Preps	250 Preps
HiPure DNA Mini Column II	50	250
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	100	2 x 250
Buffer RLC	50 ml	200 ml
Buffer PRC1	50 ml	200 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.