FlyCut® XbaI is expressed and purified from E.coli that carries the recombinant XbaI gene. The molecular weight is 24.7 kDa, with the recognition site at T^CTAGA. The reaction is conducted for 5-15 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dcm or mammalian CpG methylation, but sensitive to dam methylation.
Recognition Site
5’...T↓CTAGA...3’
3’...AGATC↑T...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JX101-01 | JX101-02 |
| FlyCut® XbaI | 1,500 units | 2×1,500 units |
| 10×FlyCut® Buffer | 500 μl | 1 ml |
| 10×DNA Loading Buffer | 1 ml | 1 ml |