FlyCut® NcoI is expressed and purified from E.coli that carries the recombinant NcoI gene. The molecular weight is 32.3 kDa, with the recognition site at C^CATGG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...C↓CATGG...3’
3’...GGTAC↑C...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JN101-01 | JN101-02 |
| FlyCut® NcoI | 500 units | 2×500 units |
| 10×FlyCut® Buffer | 250 μl | 250 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |