EasyTaq® DNA Polymerase
EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.
Applications
• Routine PCR
• High throughput PCR
• Colony PCR
Storage
at -20 ℃ for two years
Product Contents
| Component | AP111-01 | AP111-02 | AP111-03 | AP111-04 |
| EasyTaq® DNA Polymerase | 500 U×1 | 500 U×6 | 5000 U×2 | 5000 U×10 |
| 10×EasyTaq® Buffer | 1.2 ml×1 | 1.2 ml×6 | 1.2 ml×20 | 1.2 ml×100 |
| 6×DNA Loading Buffer | 1 ml×1 | 1 ml×2 | 1 ml×4 | 1 ml×20 |
Without dNTP, which is separately available.
Citations
Zhu C , Gao Y , Guo H , et al. Single-Cell 5-Formylcytosine Landscapes of Mammalian Early Embryos and ESCs at Single-Base Resolution[J]. Cell Stem Cell, 2017, 20(5):720-731.
Shao N , Jiang S , Zhang M , et al. MACRO: A Combined Microchip-PCR and Microarray System for High-throughput Monitoring of Genetically Modified Organisms[J]. Analytical Chemistry, 2013, 86(2):1269-1276.
Li J , Fu J , Chen Y , et al. The U6, Biogenesis-Like 1, Plays an Important Role in Maize Kernel and Seedling Development by Affecting the 3′ End Processing of U6 snRNA[J]. Molecular Plant, 2017, 10(3):470-482.
Simond AM , Rao T , Zuo D , et al. ErbB2-positive mammary tumors can escape PI3K-p110α loss through downregulation of the Pten tumor suppressor[J]. Oncogene, 2017, 36(43):6059-6066.
Li Y , Guo SJ , Shao N , et al. A universal multiplex PCR strategy for 100-plex amplification using a hydrophobically patterned microarray[J]. LAB ON A CHIP, 2011, 11(21):3609-8.