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The kit is designed for the isolation of genomic DNA from various fresh and dried plant samples, including plant samples rich in polysaccharides and polyphenols. The kit is based on unique extraction system and membrane technology to specifically remove secondary metabolites such as polysaccharides, polyphenols, and lipids in samples, without the need for toxic reagents such as phenol and chloroform. Purified DNA with high quality and good stability can be used for enzyme digestion, PCR, Southern Blot, etc.
Features
- Wide range of applications: applicable to various plant tissues, especially those rich in polysaccharides, polyphenols or starch
- Fast operation: high-quality genomic DNA can be extracted in less than 1 hour
- Safe and low toxicity: no phenol and chloroform
- High purity: the unique technology can efficiently remove impurities such as pigments, polyphenols and polysaccharides in the sample
When dealing with woody or uncommon samples, The HiPure Plant RNA Plus Kit (R415002) and EasyPure® Universal Plant Total RNA Kit (ER302) are recommended first. R415002 contains two polysaccharide/polyphenol lysis buffer, which are amongst the most universal reagents. While ER302 contains an DNase I solution, as well as a strong polysaccharide/polyphenol lysis buffer.
HiPure Plant RNA Mini (R415102) is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
Component |
EE112-01 (50 rxns) |
EE112-02 (200 rxns) |
Lysis Buffer 49 (LB49) | 40.5 ml | 162 ml |
Precipitation Buffer 49 (PB49) | 10.5 ml | 42 ml |
RNase A | 2×1.1 ml | 2×4.2 ml |
Clean Buffer 49 (CB49 ) | 13 ml | 52 ml |
Wash Buffer 49 (WB49 ) | 11 ml | 2×22 ml |
Elution Buffer(EB ) | 15 ml | 60 ml |
Filtration Columns with Collection Tubes | 50 | 200 |
Genomic Spin Column with Collection Tubes | 50 | 200 |
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