FlyCut® EcoRV

JE301-02

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Product Details

FlyCut^® EcoRV is expressed and purified from E.coli that carries the recombinant EcoRV gene. The molecular weight is 29.5 kDa, with the recognition site at GAT^ATC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...GAT↓ATC...3’
3’...CTA↑TAG...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents

Component	JE301-01	JE301-02
FlyCut® EcoRV	2,000 units	2×2,000 units
10×FlyCut® Buffer	500 μl	1 ml
10×DNA Loading Buffer	1 ml	1 ml