FlyCut® EagI

JE101-01

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Product Details

FlyCut^® EagI is expressed and purified from E.coli that carries the recombinant EagI gene. The molecular weight is 35.1 kDa, with the recognition site at C^GGCCG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...C↓GGCCG...3’
3’...GCCGG↑C...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents

Component	JE101-01	JE101-02
FlyCut® EagI	250 units	2×250 units
10×FlyCut® Buffer	250 μl	250 μl
10×DNA Loading Buffer	1 ml	1 ml