Product Details
A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp-20 bp on both 5’- and 3’- end, respectively, are mixed and incubated with Exnase II at 37℃ for 30 min. The cloning products can then be directly transformed to competent cells with a positive rate > 95%.
ClonExpress MultiS One Step Cloning Kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit, up to five fragments can be orderly assembled in a single reaction. In addition, Exnase MultiS is compatible with the reaction systems of restriction enzyme digestion and PCR, which means that both the digestion products of vector and the PCR products of insert can be directly used for recombination without purification, significantly simplifying the procedures.
Key Features
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High Efficiency: No ligase required, no self-ligation, with a positive cloning rate >95%.
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Multi-Fragment Compatibility: Supports 2 to 5 DNA fragments (50 bp - 10 kb) for complex vector construction.
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Restriction-Free Cloning: No need to consider restriction sites in insert sequences.
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Streamlined Workflow: Direct use of PCR products and linearized vectors without purification.
- Software Support: Includes access to online primer design software “CE Design” for efficient cloning setup.
Kit Components
- ClonExpress® MultiS One Step Cloning Kit
- Optimized reaction buffer
- Enhanced Exnase MultiS recombinase
Storage Conditions
- Store at -30°C to -15°C
- Transport at ≤0°C
- Avoid repeated freeze-thaw cycles to maintain enzyme activity
This kit is an ideal solution for complex vector construction, including multi-gene expression systems, synthetic biology applications, and advanced molecular cloning projects.
See manual for more details on C113:
Cloning competent cells can be ordered with this cloning kit. Please ask us for help if you need to add the competent cells to your order of this cloning kit.
SOC Medium and SOB Medium may be purchased with this cloning kit.