Technical Data
|
Appearance |
White amorphous lyophilized powder |
|
Protein purity |
≥95% |
|
Specific activity |
≥30 U/mg Protein |
|
Deoxyribonuclease activity |
No activity of deoxyribonuclease was detected |
|
Ribonuclease activity |
No activity of Ribonuclease was detected |
Enzymatic Properties
|
Source |
Tritirachium album |
|
|
Classification |
EC 3.4.21.64 |
|
|
Molecular weight |
29 kDa (SDS-PAGE) |
|
|
Isoelectric point |
7.81 |
|
|
Optimal pH |
7.0-12.0 Both have high activity |
Char1 |
|
Optimum temperature |
65 ℃ |
Chart2 |
|
pH stability |
pH 4.5-12.5 (25 ℃, 16 h) |
Chart3 |
Thermal stability Stable below 50 ℃（pH 8.0, 30 min） Chart4
|
Activator |
SDS, Urea |
|
Inhibitor |
Diisopropylfluorophosphate (DFIP)，Benzenesulfonyl fluoride (PMSF) |
|
Storage conditions |
Dry powder can be stored at - 20 ℃ for a long time; After dissolving, it should be packed into proper volume, stored at 2-8 ℃ for a short time, and stored at - 20 ℃ for a long time. |
Use:
The virus consists of a protein shell and an internal nucleic acid. To amplify the nucleic acid by PCR, the shell protein must be destroyed first. The role of protease K is to break down the protein shell of the virus, releasing nucleic acid for easy detection.
1. Gene diagnosis kit；2. RNA and DNA Extraction Kit；3. Extract non protein components from tissues and degrade protein impurities. For example, the preparation of DNA vaccine；4.
Preparation of chromosome DNA by pulse electrophoresis；5. Western blotting；6. R&D and mass
production of enzyme based glycated albumin reagents for in vitro diagnosis.