Size :50ul
Clone Number:4B2
Aliases:Apoptosis-inducing factor 1, mitochondrial (EC 1.1.1.-) (Programmed cell death protein 8), AIFM1, AIF PDCD8
Product Type:Recombinant Antibody
Immunogen Species:Homo sapiens (Human)
UniProt ID:O95831
Immunogen:A synthesized peptide derived from human AIF
Raised in:
Species Reactivity:Human, Rat
Tested Applications:ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200
Background:Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Clonality:Monoclonal
Isotype:Rabbit IgG
Purification Method:Affinity-chromatography
Conjugate:Non-conjugated
Buffer:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form:Liquid
Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names:AIFM1
Research Areas:Epigenetics and Nuclear Signaling; Cancer; Cell biology; Metabolism