2×TransTaq® High Fidelity (HiFi) PCR SuperMix II (+dye)

AS131-01a

Product Details

TransTaq® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA. The SuperMix is  provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

Features

• TransTaq® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.

• Extension rate is about 1-2 kb/min.

• Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.

• Amplification of genomic DNA fragment up to 15 kb.

Applications

• Complex templates

• GC/AT-rich templates

• Long PCR

• High yield PCR

Storage

at -20 ℃ for two years

Shipping

Dry ice (-70 ℃)

Product Composition

Component	AS131-01/21	AS131-02/22
2×TransTaq® HiFi PCR SuperMix (Mix I)/(Mix II)	1 ml	5×1 ml
Nuclease-free Water	1 ml	5 ml

References

1 Guo C J, Ma X K, Xing Y H, et al. Distinct processing of lncRNAs contributes to non-conserved functions in stem cells[J]. Cell, 2020.(IF 36.21)