2 × Phanta Flash Master Mix utilizes the new generation of Phanta Flash Super-Fidelity DNA Polymerase. Through the directed evolution of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of high fidelity (mismatch rate is 1/106 of conventional Taq DNA polymerase) and rapid amplification (5 sec/kb). With the application of the latest hot-start technology and the addition of specific factors, its amplification specificity and success rate are significantly improved. 2 × Phanta Flash Master Mix contains Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer. It only requires the addition of primers and templates for amplification, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. The amplification products of 2 × Phanta Flash Master Mix are blunt-ended, which are subsequently applicable for ClonExpress Ultra One Step Cloning Kit V2 (Vazyme #C116) and Ultra-Universal TOPO Cloning Kit.
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High fidelity: ~106X higher than Taq DNA Polymerase
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Flash amplification: Extension time up to 5 sec/kb
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Robust performance: Superior specificity, sensitivity, and yields
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Broad compatibility: Suitable for complex samples & GC-rich systems
