TransStart® FastPfu Fly DNA Polymerase is a hot start, high-fidelity and high processivity DNA Polymerase used for fast PCR. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate (6kb/min vs 4kb/min), featuring higher amplification efficiency, higher yield, higher fidelity and higher specificity.2×TransStart® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and TransStart® FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into pEASY®-Blunt series of vectors.
• Offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• Hot start, high specificity.
• High amplification efficiency.
• Fast and high fidelity.
• High sensitivity.
• High production.
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AP231-21-V3 | AP231-22-V3 | AP231-23-V3 |
| TransStart® FastPfu Fly DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 2×TransStart® FastPfu Fly Reaction | 1 ml×3 | 1 ml ×6 | 1 ml ×36 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl ×1 | 1 ml ×1 |
| PCR Stimulant | 200 μl×1 | 400 μl×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml ×1 | 1 ml ×2 |
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