Recombinant Human Novel Coronavirus Nucleoprotein(N)(D63G,R203M,D377Y) (Active)

Recombinant Human Novel Coronavirus Nucleoprotein(N)(D63G,R203M,D377Y) (Active)

CSB-EP3325GMY(M14)
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Size:1mg. Other sizes are also available. Please contact us.

Research Areas:Microbiology

Uniprot NO.:P0DTC9

Uniprot Entry Name:

Gene Names:N

Species:Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Source:E.coli

Expression Region:1-419aa(D63G,R203M,D377Y)

Sequence:MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEGLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSMGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKAYETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

Protein Description:Full Length

Tag Info:N-terminal 6xHis-tagged

Mol. Weight:49.1 kDa

Biological_Activity:?Measured by its binding ability in a functional ELISA. Immobilized N(CSB-EP3325GMY(M14)) at 2 ?g/ml can bind N Mouse Monoclonal Antibody(CSB-MA33255A2m), the EC50 of N Mouse Monoclonal Antibody is 3.834 - 5.294 ng/ml. ?Measured by its binding ability in a functional ELISA. Immobilized N(CSB-EP3325GMY(M14)) at 2 ?g/ml can bind N Recombinant Antibody(CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody is 1.304 - 1.875 ng/ml.

Purity:Greater than 85.7% as determined by SDS-PAGE.

Endotoxin:Not test.

Form:Lyophilized powder

Buffer:Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

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Paythway:

HGNC Database Link:

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STRING Database Link:

OMIM Database Link:

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