Recombinant Human Interleukin-21 protein(IL21) (Active)

Recombinant Human Interleukin-21 protein(IL21) (Active)

CSB-AP001881HU
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Size:100ug. Other sizes are also available. Please contact us.

Research Areas:Immunology

Uniprot NO.:Q9HBE4

Uniprot Entry Name:IL21_HUMAN

Gene Names:IL21

Species:Homo sapiens (Human)

Source:E.Coli

Expression Region:23-155aa

Sequence:QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS

Protein Description:Full Length of Mature Protein

Tag Info:Tag-Free

Mol. Weight:15.4 kDa

Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by by a cell proliferation assay using human N1186 T cells is less than 20 ng/ml, corresponding to a specific activity of ? 5.0 × 104 IU/mg.

Purity:>97% as determined by SDS-PAGE and HPLC.

Endotoxin:Less than 1.0 EU/µg as determined by LAL method.

Form:Lyophilized powder

Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Alternative Name/ Alias:IL-21,

Relevance:Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. {ECO:0000250, ECO:0000269|PubMed:11081504, ECO:0000269|PubMed:15178704}.

PubMed ID:11081504; 17673207; 15815621; 15489334; 15178704; 15147560; 17565991; 22235133; 24746753

Function:Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.

Involvement in disease:Immunodeficiency, common variable, 11 (CVID11)

Subcellular Location:Secreted

Protein Families:IL-15/IL-21 family

Tissue Specificity:Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.

Paythway:Jak-STATsignalingpathway

HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:6005

UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=567559

KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:59067

STRING Database Link:https://string-db.org/network/9606.ENSP00000264497

OMIM Database Link:https://www.omim.org/entry/605384605384605384

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