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Updated Date: Stock Protein updated on 20170405
Research areas: others
Target / Protein: dhaK
Biologically active: Not Tested
Expression system: E.coli
Species of origin: Citrobacter freundii
Delivery time: 3-7 business days
Uniprot ID: P45510
AA Sequence: ALVAGIVELVTATLSDLETHLNALDAKVGDGDTGSTFAAAAREIASLLHRQQLPLNNLATLFALIGERLTVVMGGSSGVLMSIFFTAAGQKLEQGANVVEALNTGLAQMKFYGGADEGDRTMIDALQPALTSLLAQPKNLQAAFDAAQAGAERTCLSSKANAGRASYLSSESLLGNMDPGAQRLAMVFKALAE
Tag info: N-terminal 6xHis-SUMO-tagged
Expression Region: 356-548AA
Protein length: Partial
MW: 35.9 kDa
Alternative Name(s): Glycerone kinase
Relevance: Catalyzes the phosphorylation of dihydroxyacetone.
Reference: "Crystal structure of the Citrobacter freundii dihydroxyacetone kinase reveals an eight-stranded alpha-helical barrel ATP-binding domain." Siebold C., Arnold I., Garcia-Alles L.F., Baumann U., Erni B. J. Biol. Chem. 278:48236-48244(2003)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.