ProteinExt® Mammalian Membrane Extraction Kit provides a simple and efficient method to extract membrane proteins from mammalian cells and tissues. Native proteins can be obtained within 70 minutes without ultracentrifugation. Up to 90% efficiency for membrane proteins have at least 1-2 transmembrane domains. The extracted protein is suitable for a variety of downstream applications, including SDS-PAGE, Western Blot, ELISA, and enzyme-activity assays.
Size: 50 rxn.
Up to 90% efficiency for membrane proteins have at least 1-2 transmembrane domains.
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Storage |
ProteinSafe™ Protease Inhibitor Cocktail at -20°C for one year, others at 2-8°C for one year
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Kit Content |
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Component |
DE301-01 (50 rxns) |
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Membrane Protein Extraction Buffer I (MPEB I) |
50mL |
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Membrane Protein Extraction Buffer II (MPEB II) |
7.5mL |
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Membrane Protein Extraction Buffer III (MPEB III) |
15mL |
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ProteinSafeTM Protease Inhibitor Cocktail, EDTA-free (100×) |
1mL
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