Description
The FavorFilter Endotoxin Free Plasmid Extraction Maxi Kit (Maxiprep) is designed for the quick isolation of endotoxin-free purified plasmid DNA using anion-exchange technology. This kit uses the FavorFilter Maxi Cartridge to clarify the lysate; the gravity-flow column has improved DNA binding capacity. PTR Buffer washes away the endotoxins in just one step. This kit is designed for the convenient, easy, and efficient extraction of pure plasmid DNA and makes the endotoxins less than 0.05 EU/µg DNA that is suitable for the transfection of cultured cells.
Features
- Time Saving: Remove bacterial lysates without centrifugation.
- High Purity: Endotoxin <0.05 EU/µg DNA, ideal for endotoxin, Equal to 2× CsCl gradient centrifugation method.
- Safety: Eliminate the use of phenol, chloroform, ethidium bromide, and cesium chloride; minimizing expose to hazardous materials.
Specifications
Format/Principle: Anion-exchange chromatography (Gravity-flow column)
Lysate Clarification: Filtration
Input Sample Size: 120~240 ml of bacterial culture for high/low-copy high-copy number or low-copy number plasmids.
Procedure time: <120 minutes
Plasmid or Constructs Range: 3 kbp~150 kbp
Binding Capacity: 1.5 mg/Maxi Prep Column
Applications
Transfection (for endotoxin sensitive cells); microinjection; in vitro transcription; in vitro transcription; restriction enzyme digestion.
Procedure
In the process, the cell pellet is resuspended (PM1; RNase A contained), lysed (PM2), and neutralized (PM3). Next, the filter cartridge is used to remove bacterial lysates and obtain cleared sample mixture. Then, the plasmid DNA/buffer mixture bind to the ion exchange resin inside the PM Maxi Column; the impurities are removed by PW Buffer. Finally, the purified plasmid DNA is eluted using high-salt PEL Buffer and precipitated with isopropanol for desalting.
Kit Composition
PEQ Buffer
PM1 Buffer
PM2 Buffer
PM3 Buffer
PTR Buffer
PW Buffer
PEL Buffer
RNase A (Lyophilized)
FavorFilter Maxi Cartridges
PM Maxi Columns
