FlyCut® SphI is expressed and purified from E.coli that carries the recombinant SphI gene. The molecular weight is 26.8 kDa, with the recognition site at GCATG^C. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...GCATG↓C...3’
3’...C↑GTACG...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JS701-01 | JS701-02 |
| FlyCut® SphI | 250 units | 2×250 units |
| 10×FlyCut® Buffer | 250 μl | 250 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |