FlyCut® ScaI is expressed and purified from E.coli that carries the recombinant ScaI gene. The molecular weight is 27.8 kDa, with the recognition site at AGT^ACT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...AGT↓ACT...3’
3’...TCA↑TGA...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JS401-01 | JS401-02 |
| FlyCut® ScaI | 500 units | 2×500 units |
| 10×FlyCut® Buffer | 250 μl | 250 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |