FlyCut® SacII is expressed and purified from E.coli that carries the recombinant SacII gene. The molecular weight is 32.1 kDa, with the recognition site at CCGC^GG The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
Recognition Site
5’...CCGC↓GG...3’
3’...GG↑CGCC...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JS201-01 | JS201-02 |
| FlyCut® SacII | 1,000 units | 2×1,000 units |
| 10×FlyCut® Buffer | 250 μl | 500 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |