FlyCut® SacI is expressed and purified from E.coli that carries the recombinant SacI gene. The molecular weight is 40.8 kDa, with the recognition site at GAGCT^C. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes.This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...GAGCT↓C...3’
3’...C↑TCGAG...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JS101-01 | JS101-02 |
| FlyCut® SacI | 1,000 units | 2×1,000 units |
| 10×FlyCut® Buffer | 250 μl | 500 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |