FlyCut® EcoRI

JE201-01

Product Details

FlyCut^® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...G↓AATTC...3’
3’...CTTAA↑G...5’

See manual for more details on JE201:

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C

Shipping
Ice/gel packs (4℃)

Product Contents

Component	JE201-01	JE201-02
FlyCut® EcoRI	5,000 units	2×5,000 units
10×FlyCut® Buffer	1.5 ml	3×1 ml
10×DNA Loading Buffer	0.5 ml	1 ml