6x Gel Loading Buffer with Dyes

M904

Quantity: 5 x 1 mL tubes (5 mL total)

Gel Loading Buffer with Dye (6X) is a pre-mixed Loading Buffer with two tracking dyes for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping any ongoing reaction. Bromophenol blue and xylene cyanol are the standard tracking dye for electrophoresis.

Contents
Gel Loading Dye (6X):

60 mM Tris-HCL（pH 8.0）
0.06% Bromophenol Blue
0.03% Xylene Cyanol
60% Glycerol
60 mM EDTA

Storage

Store at RT

Quality Assurance Statement
Gel Loading Dye(6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.

Dye Migrate Rate (on a standard 1% TAE or TBE agarose gel)
Xylene Cyanol
TAE: 4160 bp
TBE: 3030 bp

Bromophenol Blue
TAE: 370 bp
TBE: 220 bp